Zeiss CD7-WF
Brief description:
The Zeiss Celldiscoverer 7 is an automated fluorescent wide-field microscope capable of imaging microplates and microscope slides. It can image in a variety of magnifications and is equiped with an automated water-immersion system for the 50x objective. It is integrated in a robotic system that allows to automatically image several plates in sequence.
Microscope:
- Zeiss Celldiscoverer 7
Contrast Methods:
- Adaptive Phase Gradient Contrast (PGC), Widefield Fluorescence
Ilumination (visual):
- Calibri.2 LED Illumination: 385/420/470/520/567/590/625 nm
Beam Splitter:
- RQFT 405+493+575+653
- RTFT 450+538+610
- RTFT 405+493+61
Emission Filters:
- QBP 425/30+514/30+592/25+709/100
- TBP 467/24+555/25+687
- TBP 425/30+524/50+688
- 431/28
- 515/30
- 598/25
Objectives:
- PlanApochromat 5x/0.35/WD: 5.1 mm
- PlanApochromat 20x/0,7/WD: 2.2 mm
- PlanApochromat 20x/0,95/WD: 0.76 mm
- PlanApochromat 50x/1,2 Water | Autoimmersion/WD: 0.84 mm
These objectives are used in combination with a 0.5x, 1x and 2x optovar lens. Possible magnifications: 2.5x, 5x, 10x, 20x, 25x, 40x, 50x, 100x.
Camera/detectors:
- Axiocam 712 mono
- Hamamatsu Orca-Fusion BT
Incubation:
- The system is equipped with a temperature control and CO2 gassing system
Software:
- ZEN Blue
Key features:
- Integrated into a robotic system, can image up to 30 plates in sequence
- Hardware based autofocus
- Automated water immersion (50x)
- Integrated image analysis allowing for 'guided acquisition'
- Can be controlled by Python macros
- Barcode recognition
- Automatic calibration routines