DeltaVision OMX
Brief description:
The OMX is a fluorescence based super-resolution system capable of fast multicolour 3D structured illumination (3D-SIM) (Blaze SIM Module). Further applications comprise TIRF microscopy (Ring TIRF), fast widefield microscopy and localization microscopy (widefield or TIRF acquisition).
The system is equipped with four liquid cooled sCMOS cameras for simultaneous multi channel acquisition , a stage incubator for live cell imaging and a hardware autofocus (Ultimate focus).
Microscope:
- OMX V4 TIRF, Blaze SIM Module + Monet Package
Ilumination:
- widefield: Insight SSI
- SIM + TIRF: diode lasers, laser lines: 405, 445, 488, 514, 568, 642 nm
Objectives:
- SIM: PlanApoN 60x /1.42NA Oil PSF
- widefield: UPlanSApo 100x / 1.4NA Oil
- TIRF: ApoN 60x / 1.49NA Oil TIRF
Camera / detectors:
- 4 cameras: sCMOS OMX V4 (15bit range)
Software:
- DeltaVision OMX (3.30.4378.0)
- softWoRx 6.0 (5.9.9)
Stage:
- xyz nanomotion stage, 25 mm travel range
- piezo controlled fast z-axis, 100 µm range
Specifications:
- SIM resolution
- lateral (xy): 120–160 nm
- axial (z): 280–350 nm
- voxel size (xyz)
- 80 x 80 x 125 nm (raw data image)
- 40 x 40 x 125 nm (reconstructed image)
- max number of pixels (xy)
- 512 x 512 (raw data image)
- 1024 x 1024 (reconstructed image)