Renishaw Raman

Moreover, a set of low frequency filters is available allowing measurements 15 cm^-1 away from the laser line (532 nm laser). On top a variety of lasers (532 nm, 785 nm and 1064 nm) for Raman, LED illumunation for fluorescent imaging and a 1064 nm laser for optical trapping are available.   

Technical details

Raman system and microscope:

• InVia Qontor confocal Raman microscope with Leica DM2700 microscope body (upright) motorized stage with fluorescence filter sets L5 ET and N2.1.

Excitation source:

• Raman Lasers:
  • 532 nm Nd:YAG (200 mW)
  • 785 nm Diode laser (100 mW)
  • 1064 nm Nd:YAG (400 mW)      
• Optical Laser Trap: 1064 nm (400 mW)                
  
• HXP120 lamp for fluorescence, transmssion LED

Objectives:

• 5x 0.12NA, N-Plan WD 13.2 mm
  
• 20x 0.4NA, N-Plan WD 1.1 mm
  
• 100x 0.85NA, N-Plan WD 0.33 mm
• 50x 0.55NA, LWD Fluotar WD 8 mm
• 100x 0.75NA, LWD Fluotar WD 4.7 mm
  
• 100x 1.25NA, Oil HC FL Plan WD 0.12 mm
• 63x 0.9NA, WaterHCX PL Apo WD 0.9 mm (for optical trap)

Detectors:

Raman detectors:

• Renishaw CTX.1024A256-FDU: Peletier cooled (-70 °C) UV-NIR enhanced CCD camera (532 nm + 785 nm lasers)
  
• iDUS InGaAs 1.7 µm array detector (512 pixel array) (1064 nm laser)

cameras:

• internal colour camera for BF detection
  
• Hi-res video camera (5MP monochrome) for fluorescence detection
  

Grating for Raman spectroscopy:

• 1200 lines/mm (785 nm laser)
• 1800 lines/mm (532 nm laser)
• 3000 lines/mm (532 nm laser)
• 830 lines/mm (1064 nm laser)   
  

Software:

• WIRE 5.5

Key features:

• spectral acquisition (Rayleigh filters allow measurements to 100 cm^-1 from the laser line)
  
  • at a single position (spot)
    
  • as depth series (z-stack)
    
  • as xy map acquisition
    1. different speed/quality options: Stream Line^TM, Stream HR^TM, Stream HR^TM Rapide modes
    2. autofocus funtion for surface acquisition: FocusTrack^TM
       
  • as 3D volume acquisition
• Optical laser trap
• Polarisation Raman measurements
  
• low frequency filters for 532 nm laser (only)
  
  • Rayleigh filters with 15 cm^-1 cut-off for 532 nm laser line only (cannot be combined with polarisation)