Olympus MM

Manufacturer:
Olympus

Model name:
MM

Location:
HPM C 16.2

external page Direct booking link to PPMS

Instrument contact:

Dr. Sung Sik Lee

Lecturer at the Department of Materials

ScopeM
Otto-Stern-Weg 3
8093 Zürich
Switzerland

Brief description

This wide field fluorescence microscope can be used for automated long-term, multi-position live cell imaging. The setup includes a motorized X-Y stage, and a box-type incubator (temperature, CO₂).

LED (Lumencore SpectraX) illumination in combination with multiband chromatic mirrors and a fast emission filterwheel allow for fast multichannel acquisition. It is equipped with the ZDC2 (Z-drift compensator) - a "hardware auto-focus" solution from Olympus. There are also the options for phase contrast imaging, DIC imaging and dark-field imaging.

A full offline version of Metamorph is installed on Image Processing Computer 2 Windows 7 in HPI F17 for further analysis of images.

Technical details

Microscope:

Olympus IX 83 (inverted)

Ilumination:

LED (Lumencor SpectraX)

Objectives:

10x NA=0.3: Air, UPLANFLN, Ph1
40x NA=0.6: Air, LUCPlanFLN, Ph2
40x NA=1.3: Oil, UPLFLN40XO, DIC (BFP1)
60x NA=1.42: Oil, PLAPON60XO, DIC (BFP1)
100x NA=1.40: Oil, UPLSAPO100XO, DIC
100x NA=1.40: Oil, UPLSAPO100XOPH, Ph3

Optional

4x NA=0.16: Air, UPLSAPO
60x NA =1.25 (Iris 0.65–1.25): Oil, UPlanFLN
100x NA=1.3 (Iris 0.6–1.3): Oil, UPlanFLN

Contrast methods:

Phase contrast imaging (10X, 40X, 100X)
DIC imaging
Dark-field imaging (transmission mode)

Camera / detectors:

Hamamatsu ORCA-Flash 4.0 V2 (sCMOS, 2048 × 2048 pixels, pixel size 6.5 μm × 6.5 μm)

Software:

Metamorph 7.10.1 (Molecular Devices)

Key features:

Live Cell Imaging : Temperature, CO₂ controller, Hardware autofocusing for long-term stable focusing
Phase contast imaging, DIC imaging, Transmitted darkfield imaging