Tomocube HT-2H QPI
Brief description
Holotomographic Microscopy utilize optical diffraction tomography (ODT), which enables users to quantitatively and noninvasively investigate biological cells and tissues. ODT reconstructs the 3D refractive index (RI) distributions of live cells using a dynamic micromirror device (DMD) and by doing so, provides structural and biochemical information about the cell including dry mass, morphology, and dynamics of the cellular membrane. In addtion, the HT-2H is able to perform fluorescence microscopy.
Technical details
Microscope:
- Tomocube HT-2H
Ilumination:
- Phase imaging: 532 nm diode laser
- Fluorescence : 3 LEDs, λcenter = 385 nm, 470 nm, 570 nm
Objectives:
- 60X NA=1.2, water immersion
Key features:
- Label-free imaging
- Refractive index measurement, Quantiative phase imaging
- Full motorized stage (X, Y, Z axis)
- Live Cell Imaging : Temperature, CO2 controller
Technical Specification:
- Lateral resolution: 110 nm
- Axial resolution 345 nm
- FOV: 80 μm DOF: 40 μm
- Time resolution: 150 fps (2D), 2.5 fps (3D)
- Max exposure time: 1sec
Software:
- Tomostudio 3.3.3 (Tomocube)
- Tomosticher
Link:
Fluorescence settings: