Expansion Microscopy

2025

• external page Ultrastructure Expansion Microscopy (U-ExM) of the Fission Yeast Schizosaccharomyces pombe Felix Mikus and Gautam Dey

• external page Plants in the expansion era: Recent advances in expansion microscopy in plant systems Kevin L Cox,Kirk J Czymmek

2024

• external page One step 4x and 12x 3D-ExM: robust super-resolution microscopy in cell biology external page Roshan X Norman,Yu-Chia Chen,Emma E Recchiaexternal page , Jonathan Loi,Quincy Rosemarie,Sydney L Lesko,Smit Patel, Nathan Sherer, Motoki Takaku,Mark E Burkard,Aussie Suzuki

• external page Subdiffraction Imaging of Cleared and Expanded Large-Scale Tissue Yawen Zhang,Weiyue Wu,Hongdou Shen,Juan Xu, Qing Jian,Xiaodong Han, Pingqiang Cai

• external page Derivative Technologies of Expansion Microscopy and Applications in Biomedicine Dongling Jia,Minhui Cui,Adeleh Divsalar,Tawfik A Khattab,Salhah D Al-Qahtani,Edwin Cheung,Xianting Ding

• external page Expanding boundaries - a cell biologist's guide to expansion microscopy Nadja Hümpfer, Ria Thielhorn, Helge Ewers

• external page EDTP enhances and protects the fluorescent signal of GFP in cleared and expanded tissues Ruili Feng, Jiongfang Xie, Liang Gao

• external page Expansion microscopy reveals characteristic ultrastructural features of pathogenic budding yeast species Md Hashim Reza, Srijana Dutta, Rohit Goyal, Hiral Shah, Gautam Dey, Kaustuv Sanyal

• external page Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures Ewa Langner, Pongpratch Puapatanakul, Rachel Pudlowski, Dema Yaseen Alsabbagh, Jeffrey H Miner, Amjad Horani, Susan K Dutcher, Steven L Brody, Jennifer T Wang, Hani Y Suleiman, Moe R Mahjoub

2023

• external page Universal Molecular Retention with 11-Fold Expansion Microscopy Brendan R Gallagher, Aleksandra Klimas, Zhangyu Cheng, Yongxin Zhao

• external page An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) of Chlamydomonas reinhardtii Nikolai Klena, Giovanni Maltinti, Umut Batman, Gaia Pigino, Paul Guichard, Virginie Hamel

• external page Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging external page Samia Parveen,Nicolas W Jones, Ian Millerschultz, external page Adam C Paré

• external page Current Progress in Expansion Microscopy: Chemical Strategies and Applications Gang Wen,Volker Leen,Taoufik Rohand,Markus Sauer,Johan Hofkens

• external page Expansion Microscopy: Super-Resolution Imaging with Hydrogels Sven Truckenbrodt

• external page Expansion microscopy: A chemical approach for super-resolution microscopy external page Yinyin ZhuangXiaoyu Shi

• external page Protein and lipid expansion microscopy with trypsin and tyramide signal amplification for 3D imaging Ueh-Ting Tim Wang, Xuejiao Tian, Yae-Huei Liou, Sue-Ping Lee, Hsiao-Tang Hu, Chieh-Han Lu, Po-Ting Lin, Ya-Jen Cheng, Peilin Chen, Bi-Chang Chen    

• external page Magnify is a universal molecular anchoring strategy for expansion microscopy Aleksandra Klimas, Brendan R Gallagher, Piyumi Wijesekara, Sinda Fekir, Emma F DiBernardo, Zhangyu Cheng, Donna B Stolz, Franca Cambi, Simon C Watkins, Steven L Brody, Amjad Horani, Alison L Barth, Christopher I Moore, Xi Ren, Yongxin Zhao

• external page iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy Vincent Louvel , Romuald Haase , Olivier Mercey , Marine H Laporte , Thibaut Eloy , Étienne Baudrier , Denis Fortun , Dominique Soldati-Favre , Virginie Hamel , Paul Guichard

2022

• external page Visualizing proteins by expansion microscopy Ali H. Shaib, Abed Alrahman Chouaib, Rajdeep Chowdhury, Daniel Mihaylov, Chi Zhang, Vanessa Imani, Svilen Veselinov Georgiev, Nikolaos Mougios, Mehar Monga, Sofiia Reshetniak, Tiago Mimoso, Han Chen, Parisa Fatehbasharzad, Dagmar Crzan, Kim-Ann Saal, Nadia Alawar, Janna Eilts, Jinyoung Kang, Luis Alvarez, Claudia Trenkwalder, Brit Mollenhauer, Tiago F. Outeiro, Sarah Köster, Julia Preobraschenski, Ute Becherer, Tobias Moser, Edward S. Boyden, A Radu Aricescu, Markus Sauer, Felipe Opazo, Silvio O. Rizzoli

• external page Expansion microscopy with ninefold swelling (NIFS) hydrogel permits cellular ultrastructure imaging on conventional microscope Hongxia Li,Antony R Warden, Jie He, Guangxia Shen, Xianting Ding

• external page Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast Kerstin Hinterndorfer, Marine H Laporte, Felix Mikus, Lucas Tafur, Clélia Bourgoint, Manoel Prouteau, Gautam Dey, Robbie Loewith, Paul Guichard, Virginie Hamel

2021

• external page Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy. Paul Tillberg

• external page Tetra-gel enables superior accuracy in combined super-resolution imaging and expansion microscopy. Hsuan Lee, Chih-Chieh Yu, Edward S Boyden, Xiaowei Zhuang, Pallav Kosuri

• external page Ultrastructure expansion microscopy (U-ExM). Davide Gambarotto, Virginie Hamel, Paul Guichard

• external page Expansion microscopy of the mitotic spindle. Ivana Ponjavić, Kruno Vukušić2, Iva M Tolić

• external page Expansion STED microscopy (ExSTED). Mengfei Gao, Ria Thielhorn, Jakob Rentsch, Alf Honigmann, Helge Ewers

2020

• external page Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM) Fabian U Zwettler, Sebastian Reinhard, Davide Gambarotto, Toby D M Bell, Virginie Hamel, Paul Guichard, Markus Sauer

• external page Simple multi-color super-resolution by X10 microscopy. Sven Truckenbrodt, Silvio O Rizzoli

• external page Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells. Chi Zhang, Jeong Seuk Kang, Shoh M Asano, Ruixuan Gao, Edward S Boyden

• external page Fluorescence Photobleaching as an Intrinsic Tool to Quantify the 3D Expansion Factor of Biological Samples in Expansion Microscopy Marisa Vanheusden, Raffaele Vitale, Rafael Camacho, Kris P F Janssen, Aline Acke, Susana Rocha, Johan Hofkens

• external page Expansion microscopy of C. elegans

• external page An introduction to the methodology of expansion microscopy. Emma L Faulkner, Steven G Thomas, Robert K Neely Chih-Chieh (Jay) Yu, Nicholas C Barry, Asmamaw T Wassie, Anubhav Sinha, Abhishek Bhattacharya, Shoh Asano2, Chi Zhang, Fei Chen, Oliver Hobert, Miriam B Goodman, Gal Haspel, Edward S Boyden

2019

• external page Expansion microscopy for the analysis of centrioles and cilia. Sahabandu N, Kong D, Magidson V, Nanjundappa R, Sullenberger C, Mahjoub MR, Loncarek

• external page Basics of Expansion Microscopy. Klimas A, Gallagher B, Zhao Y

• external page Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions. Jaiswal A, Hoerth CH, Zúñiga Pereira AM, Lorenz H.

• external page Expansion Microscopy: Scalable and Convenient Super-Resolution Microscopy. Tillberg PW, Chen F.

• external page A practical guide to optimization in X10 expansion microscopy. Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG.

• external page Imaging cellular ultrastructures using expansion microscopy (U-ExM). Gambarotto D, Zwettler FU, Le Guennec M, Schmidt-Cernohorska M, Fortun D, Borgers S, Heine J, Schloetel JG, Reuss M, Unser M, Boyden ES, Sauer M, Hamel V, Guichard P.

• external page Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution. Gao R, Asano SM, Upadhyayula S, Pisarev I, Milkie DE, Liu TL, Singh V, Graves A, Huynh GH, Zhao Y, Bogovic J, Colonell J, Ott CM, Zugates C, Tappan S, Rodriguez A, Mosaliganti KR, Sheu SH, Pasolli HA, Pang S, Xu CS, Megason SG, Hess H, Lippincott-Schwartz J, Hantman A, Rubin GM, Kirchhausen T, Saalfeld S, Aso Y, Boyden ES, Betzig E.

• external page Multiplexed expansion microscopy of the brain through fluorophore screening. Min K, Cho I, Choi M, Chang JB.

• external page Amplified Expansion Stimulated Emission Depletion Microscopy. Kim D, Kim T, Lee J, Shim SH.

 

• external page Rapid high resolution 3D imaging of expanded biological specimens with lattice light sheet microscopy. Tsai YC, Tang WC, Low CSJ, Liu YT, Wu JS, Lee PY, Chen LQ, Lin YL, Kanchanawong P4, Gao L, Chen BC.
  

2018

• external page Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues. Asano SM, Gao R, Wassie AT, Tillberg PW, Chen F, Boyden ES.

• external page X10 expansion microscopy enables 25-nm resolution on conventional microscopes.Truckenbrodt S, Maidorn M, Crzan D, Wildhagen H, Kabatas S, Rizzoli SO.

• external page Expansion microscopy: enabling single cell analysis in intact biological systems. Alon S, Huynh GH, Boyden ES.

• external page Expansion microscopy. Cho I, Seo JY, Chang J.

• external page Expansion enhanced nanoscopy. Li R, Chen X, Lin Z, Wang Y, Sun Y.

• external page Superresolution imaging of Drosophila tissues using expansion microscopy. Jiang N, Kim HJ, Chozinski TJ, Azpurua JE, Eaton BA, Vaughan JC, Parrish JZ.

• external page Combined expansion microscopy with structured illumination microscopy for analyzing protein complexes. Wang Y, Yu Z, Cahoon CK, Parmely T, Thomas N, Unruh JR, Slaughter BD, Hawley RS.

• external page Expansion Stimulated Emission Depletion Microscopy (ExSTED). Gao M, Maraspini R, Beutel O, Zehtabian A, Eickholt B, Honigmann A, Ewers H.

• external page Nanoscale Imaging of Kidney Glomeruli Using Expansion Pathology. Bucur O, Zhao Y.
  

2017

• external page Hybrid Structured Illumination Expansion Microscopy Reveals Microbial Cytoskeleton Organization. Halpern AR, Alas GCM, Chozinski TJ, Paredez AR, Vaughan JC.

• external page Confocal super-resolution imaging of the glomerular filtration barrier enabled by tissue expansion. Unnersjö-Jess D, Scott L, Sevilla SZ, Patrakka J, Blom H, Brismar H.

• external page Iterative expansion microscopy. Chang JB, Chen F, Yoon YG1, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, Cai D, Boyden ES.
  

2016

• external page Nanoscale imaging of RNA with expansion microscopy. Chen F, Wassie AT, Cote AJ, Sinha A, Alon S, Asano S, Daugharthy ER, Chang JB, Marblestone A, Church GM, Raj A, Boyden ES.

• external page Expansion microscopy with conventional antibodies and fluorescent proteins. Chozinski TJ, Halpern AR, Okawa H, Kim HJ, Tremel GJ, Wong RO, Vaughan JC.

• external page Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Tillberg PW, Chen F, Piatkevich KD, Zhao Y, Yu CC, English BP, Gao L, Martorell A, Suk HJ, Yoshida F, DeGennaro EM, Roossien DH, Gong G, Seneviratne U, Tannenbaum SR, Desimone R, Cai D, Boyden ES.

2015

• external page Optical imaging. Expansion microscopy. Chen F, Tillberg PW, Boyden ES.