Labeling, Mounting and Fluorescent Proteins
2021
- external page Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells. Hanieh Mazloom-Farsibaf, Farzin Farzam, Mohamadreza Fazel, Michael J Wester, Marjolein B M Meddens, Keith A Lidke
- external page Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels. Jonathan B Grimm, Luke D Lavis
2020
- external page Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy. Kwon J, Park JS, Kang M, Choi S, Park J, Kim GT, Lee C, Cha S, Rhee HW, Shim SH.
- external page Fluorescent Labeling of Proteins of Interest in Live Cells: Beyond Fluorescent Proteins. Ji Liu, Zongqiang Cui
- external page Super-Beacons: Open-Source Probes With Spontaneous Tuneable Blinking Compatible With Live-Cell Super-Resolution Microscopy. Pereira PM, Gustafsson N, Marsh M, Mhlanga MM, Henriques R.
- external page Visualizing and Manipulating Biological Processes Using HaloTag and SNAP-Tag Technologies. Hoelzel CA, Zhang X.
2019
- external page Troubleshooting and using controls in IHC and ICC
- external page Rational Design of Fluorogenic and Spontaneously Blinking Labels for Super-Resolution Imaging. Zheng Q, Ayala AX, Chung I, Weigel AV, Ranjan A, Falco N, Grimm JB, Tkachuk AN, Wu C, Lippincott-Schwartz J, Singer RH, Lavis LD
- external page Labeling Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags. Erdmann RS, Baguley SW, Richens JH, Wissner RF, Xi Z, Allgeyer ES, Zhong S, Thompson AD, Lowe N, Butler R, Bewersdorf J, Rothman JE, St Johnston D, Schepartz A, Toomre D.
2018
- external page Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy. Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, Richter F, Coy-Vergara J, Coceano G, Boyden ES, Duncan RR, Hell SW, Lauterbach MA, Lehnart SE, Moser T, Outeiro TF, Rehling P, Schwappach B, Testa I, Zapiec B, Rizzoli SO
- external page Fluorogenic probes for super-resolution microscopy. Kozma E, Kele P.
- external page Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy. Wang L, Frei MS, Salim A, Johnsson K.
- external page Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies. Klein A, Hank S, Raulf A, Joest EF, Tissen F, Heilemann M, Wieneke R, Tampé R.
- external page Advances in the design of cell-permeable fluorescent probes for applications in live cell imaging. Alamudi SH, Chang YT.
2017
2016
- external page A proposal for validation of antibodies. Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto T.
- external page PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins. Leyton-Puig D, Kedziora KM, Isogai T, van den Broek B, Jalink K1, Innocenti M.
2014
- external page An Introduction to Antibodies and Their Applications
- external page Advances in fluorescence labeling strategies for dynamic cellular imaging. Kevin M Dean& Amy E Palmer
2013
- external page Immunohistochemical Staining Methods
- external page Determination of the lowest concentrations of aldehyde fixatives for completely fixing various cellular structures by real-time imaging and quantification. Zeng F, Yang W, Huang J, Chen Y, Chen Y.
2012
- external page Immunolabeling artifacts and the need for live-cell imaging. Schnell U, Dijk F, Sjollema KA, Giepmans BN.
- external page Basic immunocytochemistry for light microscopy. Brooks SA.
2011
2010
- external page Labels and probes for live cell imaging: overview and selection guide. Hilderbrand SA.
- external page Antibody validation. Bordeaux J, Welsh A, Agarwal S, Killiam E, Baquero M, Hanna J, Anagnostou V, Rimm D.
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2024
- external page Spinning Disk Confocal Microscopy for Optimized and Quantified Live Imaging of 3D Mitochondrial Network Somaieh Ahmadian, Patrick J Lindsey, Hubert J M Smeets, Florence H J van Tienen, Marc A M J van Zandvoort
- external page The DNA-PAINT palette: a comprehensive performance analysis of fluorescent dyes Philipp R Steen, Eduard M Unterauer, Luciano A Masullo, Jisoo Kwon, Ana Perovic, Kristina Jevdokimenko, Felipe Opazo, Eugenio F Fornasiero, Ralf Jungmann
- external page Surface-Functionalized Halo-Tag Gold Nanoprobes for Live-Cell Long-Term Super-Resolution Imaging of Endoplasmic Reticulum Dynamics Peng Xu, Simei Zhong, Yunfei Wei, Xinxin Duan, Meng Zhang, Wei Shen, Yan Ma, Yu-Hui Zhang
2023
- external page Long-term super-resolution inner mitochondrial membrane imaging with a lipid probe Shuai Zheng, Neville Dadina, Deepto Mozumdar, Lauren Lesiak, Kayli N Martinez, Evan W Miller, Alanna Schepartz
- external page Fluorescent Probes for Lipid Membranes: From the Cell Surface to Organelles Andrey S Klymchenko
2021
2020
2019
- external page 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics. Wade OK, Woehrstein JB, Nickels PC, Strauss S, Stehr F, Stein J, Schueder F, Strauss MT1,Ganji M, Schnitzbauer J, Grabmayr H, Yin P, Schwille P, Jungmann R.
- external page Super-resolution labelling with Action-PAINT. Liu N, Dai M, Saka SK, Yin P.
- external page A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Wang C, Taki M, Sato Y, Tamura Y, Yaginuma H, Okada Y, Yamaguchi S.
- external page MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience. Collot M, Ashokkumar P, Anton H, Boutant E, Faklaris O, Galli T, Mély Y, Danglot L, Klymchenko AS.
2018
- external page Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues. Lukinavičius G, Mitronova GY, Schnorrenberg S, Butkevich AN, Barthel H, Belov VN, Hell SW
- external page Fix Your Membrane Receptor Imaging: Actin Cytoskeleton and CD4 Membrane Organization Disruption by Chemical Fixation. Pereira PM, Albrecht D, Culley S, Jacobs C, Marsh M, Mercer J, Henriques R.
- external page Accelerated FRET-PAINT microscopy. Lee J, Park S, Hohng S.
- external page DNA-Based Super-Resolution Microscopy: DNA-PAINT. Nieves DJ, Gaus K, Baker MAB.
2017
- external page Actin visualization at a glance. Melak M, Plessner M, Grosse R
- external page Super-resolution microscopy with DNA-PAINT Schnitzbauer J, Strauss MT, Schlichthaerle T, Schueder F, Jungmann R
- external page Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT. Schueder F, Lara-Gutiérrez J, Beliveau BJ, Saka SK, Sasaki HM, Woehrstein JB, Strauss MT, Grabmayr H, Yin P, Jungmann R
- external page Direct fluorescent-dye labeling of α-tubulin in mammalian cells for live cell and superresolution imaging. Schvartz T, Aloush N, Goliand I, Segal I, Nachmias D, Arbely E, Elia N
- external page Fluorogenic Labeling Strategies for Biological Imaging. Chenge Li, Alison G. Tebo and Arnaud Gautier
- external page Accelerated super-resolution imaging with FRET-PAINT. Jongjin Lee, Sangjun Park, Wooyoung Kang and Sungchul Hohng
- external page Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions. Yubing Han, Meihua Li, Fengwu Qiu, Meng Zhang & Yu-Hui Zhang
- external page Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal. Nina G. Bozhanova, Mikhail S. Baranov, Natalia V. Klementieva, Karen S. Sarkisyan, Alexey S. Gavrikov, Ilia V. Yampolsky, Elena V. Zagaynova, Sergey A. Lukyanov, abd Konstantin A. Lukyanov and Alexander S. Mishin
- external page Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. Mark-Anthony Bray, Shantanu Singh, Han Han, Chadwick T Davis, Blake Borgeson, Cathy Hartland, Maria Kost-Alimova, Sigrun M Gustafsdottir, Christopher C Gibson & Anne E Carpenter
- external page A Molecular Probe for the Detection of Polar Lipids in Live Cells. Christie A. Bader, Tetyana Shandala1, Elizabeth A. Carter, Angela Ivask3, Taryn Guinan, Shane M. Hickey, Melissa V. Werrett, Phillip J. Wright, Peter V. Simpson, Stefano Stagni, Nicolas H. Voelcker, Peter A. Lay, Massimiliano Massi, Sally E. Plush, Douglas A. Brooks
2016
- external page PFA fixation enables artifact-free super-resolution imaging of the actin cytoskeleton and associated proteins. Daniela Leyton-Puig, Katarzyna M. Kedziora, Tadamoto Isogai, Bram van den Broek, Kees Jalink and Metello Innocenti
- external page Fluorogenic Probes for Multicolor Imaging in Living Cells. Lukinavičius G, Reymond L, Umezawa K, Sallin O, D'Este E, Göttfert F, Ta H, Hell SW, Urano Y, Johnsson K
- external page High-density three-dimensional localization microscopy across large volumes. Wesley R Legant, Lin Shao, Jonathan B Grimm, Timothy A Brown, Daniel E Milkie, Brian B Avants, Luke D Lavis1 & Eric Betzig
- external page Live-cell protein labelling with nanometre precision by cell squeezing. Alina Kollmannsperger, Armon Sharei, Anika Raulf, Mike Heilemann, Robert Langer, Klavs F. Jensen, Ralph Wieneke & Robert Tampe
2015
2014
- external page Comparative analysis of tools for live cell imaging of actin network architecture. Belin BJ, Goins LM, Mullins RD.
- external page Fluorogenic probes for live-cell imaging of the cytoskeleton. Lukinavičius G, Reymond L, D'Este E, Masharina A, Göttfert F, Ta H, Güther A, Fournier M, Rizzo S, Waldmann H, Blaukopf C, Sommer C, Gerlich DW, Arndt HD, Hell SW, Johnsson K.
2013
- external page A review of reagents for fluorescence microscopy of cellular compartments and structures, part I: BacMam labeling and reagents for vesicular structures. Dolman NJ1, Kilgore JA, Davidson MW.
- external page A review of reagents for fluorescence microscopy of cellular compartments and structures, Part II: reagents for non-vesicular organelles. Kilgore JA1, Dolman NJ, Davidson MW.
- external page A review of reagents for fluorescence microscopy of cellular compartments and structures, Part III: reagents for actin, tubulin, cellular membranes, and whole cell and cytoplasm. Kilgore JA1, Dolman NJ, Davidson MW.
- external page Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue. Gerdes MJ, Sevinsky CJ, Sood A, Adak S, Bello MO, Bordwell A, Can A, Corwin A, Dinn S, Filkins RJ, Hollman D, Kamath V, Kaanumalle S, Kenny K, Larsen M, Lazare M, Li Q, Lowes C, McCulloch CC, McDonough E, Montalto MC, Pang Z, Rittscher J, Santamaria-Pang A, Sarachan BD, Seel ML, Seppo A, Shaikh K, Sui Y, Zhang J, Ginty F.
2010
- external page Illuminating the ubiquitin/proteasome system. Salomons FA, Acs K, Dantuma NP.
- external page Enhanced fluorescence imaging of live cells by effective cytosolic delivery of probes Massignani M, Canton I, Sun T, Hearnden V, Macneil S, Blanazs A, Armes SP, Lewis A, Battaglia G.
- external page Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system. Jones CT, Catanese MT, Law LM, Khetani SR, Syder AJ, Ploss A, Oh TS, Schoggins JW, MacDonald MR, Bhatia SN, Rice CM.
2005
2019
2016
- external page Quantitative assessment of fluorescent proteins. Paula J Cranfill, Brittney R Sell, Michelle A Baird, John R Allen, Zeno Lavagnino, H Martijn de Gruiter, Gert-Jan Kremers, Michael W Davidson, Alessandro Ustione & David W Piston
- Intracellular Photoactivation and Quantification Using Fluorescence Microscopy: Chemical Tools and Imaging Approaches. Giovanni Bassolino and Pablo Rivera-Fuentes
2015
- external page Novel uses of fluorescent proteins. Mishin AS, Belousov VV, Solntsev KM, Lukyanov KA
- external page High-performance probes for light and electron microscopy. Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern A, Pfeiffer BD, Hooks BM, Li W1, English BP, Tian T, Henry GL, Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger LL
2014
- external page Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells. Mahen R, Koch B, Wachsmuth M, Politi AZ, Perez-Gonzalez A, Mergenthaler J, Cai Y, Ellenberg J
- external page Fluorescent protein applications in microscopy. Hama H, Kurokawa H, Kawano H, Ando R, Shimogori T, Noda H, Fukami K, Johnson WL, Straight AF.
- external page Fluorescent proteins for quantitative microscopy: Important properties and practical evaluation. Shaner NC.
2012
2011
- external page Modern fluorescent proteins: from chromophore formation to novel intracellular applications. Stepanenko OV, Stepanenko OV, Shcherbakova DM, Kuznetsova IM, Turoverov KK, Verkhusha VV.
- external page A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Brakemann T, Stiel AC, Weber G, Andresen M, Testa I, Grotjohann T, Leutenegger M, Plessmann U, Urlaub H, Eggeling C, Wahl MC, Hell SW, Jakobs S.
2010
2009
- external page Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges. Wiedenmann J, Oswald F, Nienhaus GU
- external page The structure and function of fluorescent proteins. Sample V, Newman RH, Zhang J.
- external page The fluorescent protein palette: tools for cellular imaging. Day RN, Davidson MW.
- external page Cell culture medium affects GFP photostability: a solution. Bogdanov AM, Bogdanova EA, Chudakov DM, Gorodnicheva TV, Lukyanov S, Lukyanov KA.
2008
2006
- external page Detection and quantification of protein-microtubules interactions using green fluorescent protein photoconversion. Brunet S, Zimmermann T, Reynaud EG, Vernos I, Karsenti E, Pepperkok R.
- external page Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum. Keppler A, Arrivoli C, Sironi L, Ellenberg J.
- external page The dark side of EGFP: defective polyubiquitination. Baens M, Noels H, Broeckx V, Hagens S, Fevery S, Billiau AD, Vankelecom H, Marynen P.
2005
- external page A guide to choosing fluorescent proteins. Shaner NC, Steinbach PA, Tsien RY.
- external page Innovation: Photoactivatable fluorescent proteins. Lukyanov KA, Chudakov DM, Lukyanov S, Verkhusha VV.
- external page Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells. Wadsworth P, Rusan NM, Tulu US, Fagerstrom C.
2023
2019
2018
- external page Observing DNA in live cells. Weiss LE, Naor T, Shechtman Y
- external page Chromatin imaging and new technologies for imaging the nucleome. Szydlowski NA, Go JS, Hu YS.
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- external page High-resolution 3D imaging of fixed and cleared organoids. Dekkers JF, Alieva M, Wellens LM, Ariese HCR, Jamieson PR, Vonk AM, Amatngalim GD, Hu H, Oost KC, Snippert HJG, Beekman JM, Wehrens EJ, Visvader JE5, Clevers H, Rios AC.
- external page FACT or PACT: A Comparison between Free-Acrylamide and Acrylamide-Based Passive Sodium Dodecyl Sulfate Tissue Clearing for whole Tissue Imaging. Wang H, Khoradmehr A, Tamadon A
2018
- external page A qualitative comparison of ten tissue clearing techniques Orlich M, Kiefer F
- external page Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2. Meng-Tsen Ke and Takeshi Imai
- external page A Versatile Optical Clearing Protocol for Deep Tissue Imaging of Fluorescent Proteins in Arabidopsis thaliana. Musielak TJ, Slane D, Liebig C, Bayer M.
- external page Shrinkage-mediated imaging of entire organs and organisms using uDISCO. Pan C, Cai R, Quacquarelli FP, Ghasemigharagoz A, Lourbopoulos A, Matryba P, Plesnila N, Dichgans M, Hellal F, Ertürk A
2015
- external page Clarifying Tissue Clearing. Richardson DS, Lichtman JW
- external page ScaleS: an optical clearing palette for biological imaging. Hiroshi Hama, Hiroyuki Hioki, Kana Namiki, Tetsushi Hoshida, Hiroshi Kurokawa, Fumiyoshi Ishidate, Takeshi Kaneko, Takumi Akagi, Takashi Saito5, Takaomi Saido & Atsushi Miyawaki
- external page Ultramicroscopy: development and outlook. Hans-Ulrich Dodt, Saiedeh Saghafi, Klaus Becker, Nina Jährling, Christian Hahn, Marko Pende, Martina Wanis, Axel Niendorf
2014
2011