Dr. Miriam Susanna Lucas-Droste

Lecturer at the Department of Materials

ETH Zürich

ScopeM

HPM C 57.2

Otto-Stern-Weg 3

8093 Zürich

Switzerland

Additional information

Course Catalogue

Spring Semester 2025

Number	Unit
327-2144-00L	Microscopy Training Cryogenic Electron Microscopy

ScopeM related details

Expertise

Correlative light and electron microscopy (CLEM)
Electron microscopy for live sciences and soft materials: (cryo-)SEM, TEM, and volume EM (FIB-SEM, SBF-SEM and array tomography)
Establishment and adaption of respective sample preparation methods for above mentioned imaging techniques
3D illustration and modelling of volume EM data

Instrument Responsibilities at ScopeM

Leica Freeze-Substitution (HPM A56.2)
PELCO BioWave and chemistry lab in general (HPM A56.1)
Ultramicromes
- Leica EM FC6 Cryo (HPM C58)
- Leica EM UC7 (HPM C56)
- Ultracut E (HPM C56)
TFS Quanta 250 3VIEW (HPT C10)
TFS Morgani 268 (HPM A 51.1)
Staining lab (HPM A58.2)

Biography

Publications

Heiligenstein, X. & Lucas, M. S. (2022): One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM. Front. Cell Dev. Biol (10); http://dx.doi.org/10.3389/fcell.2022.866472

Reyes, C., Sajó, Z., Lucas, M. S., et al. (2022): Cocultivation of White-Rot Fungi and Microalgae in the Presence of Nanocellulose. Microbiol. Spectr. (10); http://dx.doi.org/doi:10.1128/spectrum.03041-22

Tran, H. T., Lucas, M. S., Ishikawa, T., et al. (2021): A Compartmentalized Neuronal Cell-Culture Platform Compatible With Cryo-Fixation by High-Pressure Freezing for Ultrastructural Imaging. Front. Neurosci. (15); http://dx.doi.org/10.3389/fnins.2021.726763

Lucas, M. S., Gunthert, M., Bittermann, et al. (2017): Correlation of live-cell imaging with volume scanning electron microscopy. Methods Cell Biol. (140); http://dx.doi.org/10.1016/bs.mcb.2017.03.001

Burkhardt, M. A., et al. (2017): Clot-entrapped blood cells in synergy with human mesenchymal stem cells create a pro-angiogenic healing response, Biomater. Sci.(5); http://dx.doi.org/10.1039/C7BM00276A

Spyrogianni, A., Herrmann, I. K., Lucas, M. S., et al. (2016): Quantitative analysis of the deposited nanoparticle dose on cell cultures by optical absorption spectroscopy. Future Med. (11); http://dx.doi.org/10.2217/nnm-2016-0243

Walter, K. M., et al. (2014): Hif-2alpha promotes degradation of mammalian peroxisomes by selective autophagy. Cell Metab. (20); http://dx.doi.org/10.1016/j.cmet.2014.09.017