Zeiss CD7-LSM
Brief description:
The Zeiss Celldiscoverer 7 is an automated fluorescent wide-field and confocal microscope capable of imaging microplates and microscopy slides. It is equiped with an automated water-immersion system for the 50x objective. It is integrated in a robotic system with hotels at room-temperature and an automated incubator. That allows to automatically image several plates in sequence or make long-term live-cell experiments.
Microscope:
- Zeiss Celldiscoverer 7
Contrast Methods:
- Adaptive Phase Gradient Contrast (PGC), Widefield Fluorescence
Ilumination (visual):
- Calibri.2 LED Illumination: 385/420/470/520/567/590/625 nm
- LSM 900 Lasermodul LM URGB (D)
- Diodelaser 405 nm, 5 mW,
- Diodelaser 488 nm, 10 mW,
- Diodelaser (SHG) 561 nm, 10 mW,
- Diodelaser 640 nm, 5 mW,
Beam Splitter:
- RQFT 405+493+575+653
- RTFT 450+538+610
- RTFT 405+493+610
Emission Filters:
- QBP 425/30+514/30+592/25+709/100
- TBP 467/24+555/25+687
- TBP 425/30+524/50+688
- 431/28
- 515/30
- 598/25
Objectives:
- PlanApochromat 5x/0.35/WD: 5.1 mm
- PlanApochromat 20x/0,7/WD: 2.2 mm
- PlanApochromat 20x/0,95/WD: 0.76 mm
- PlanApochromat 50x/1,2 Water | Autoimmersion/WD: 0.84 mm
These objectives are used in combination with a 0.5X, 1x and 2x optovar lens. Possible magnifications: 2.5x, 5x, 10x, 20x, 25x, 40x, 50x, 100x.
Camera / detectors:
- Axiocam 712 mono
- LSM 900 Scanmodul: 2 Gallium Arsenide Phosphid-PMT (GaAsP-PMT)
- LSM 900 Airyscan 2 Detector (32 GaAsP-PMT array for super-resolution)
Incubation:
- The microsoce is equipped with a temperature control and CO2 gassing system.
Software:
- ZEN Blue
Key features:
- Integrated into a robotic system with an incubator, allows to re-image plates over a extendet time period
- Hardware based autofocus
- Automated water immersion (50x)
- Integrated image analysis allowing for 'guided acquisition'
- Can be controlled by Python macros
- Superresolution (Airyscan 2)
- Barcode recognition
- Automatic calibration routines