Zeiss Gemini SEM 560 Volutome
Manufacturer:
Zeiss
Model name:
Gemini SEM 560
Location:
HPM A73.2 and A73.3
Phone:
2 39 44
Brief description
The Zeiss GeminiSEM 560 is an ultra-high resolution FE-SEM. The Gemini 3 column allows field-free imaging of materials and life science samples with sub 1 kV resolution below 1 nm. It features variable pressure (VP) modes to enable imaging of non-conducting and vacuum-sensitive samples.
The connected ATLAS system enables automatic scans over large areas only limited by the microscope stage and available image memory. Single images can have a maximum of 32k x 32k pixels. The Volutome module provides SBF-SEM for 3D ultrastructure imaging.
Technical details
Key features:
- High Voltage: 0.02–30 kV
- Emitter: Schottky Type
- Gemini 3 electron optics
- ATLAS mosaic tool
Detectors and vacuum modes:
- Inlens and Everhard Thornley SE2 detectors (secondary electron detectors)
- C2D: Current cascade detector for detecting SE signal under variable pressure
- aBSD1: 5 sector annular solid state backscatter electron detector
- Volume BSD: Backscatter electron detector dedicated for low kV imaging
Volutome
This exchange stage holds an in-chamber ultramicrotome to enable SBF-SEM for ultrastructure imaging of biological, resin-embedded samples in 3D. Focal charge compensation (FCC) and a dedicated detection system (volume BSD) enable optimum imaging at high vacuum conditions at very fast scan time and large fields of view. Image stacks with 3–5 nm imaging resolution, up to 20k × 20k pixels wide, and a section thickness between 20–200 nm can be acquired routinely. A special sample preparation is required for optimum results.
Booking rules
The general ScopeM booking rules apply. Exceptions for this system:
- currently no limitation for bookings to allow stack acquisition
- bookings exceeding 48 h should be coordinated with the instrument responsible