Zeiss LSM 780 upright

Manufacturer:
Zeiss

Model name:
LSM 780 upright

Location:
HPM C16.5

Phone:
3 90 53

external page Direct booking link to PPMS

Instrument contact:

Dr. Cecilia Bebeacua

Lecturer at the Department of Materials

ScopeM
Otto-Stern-Weg 3
8093 Zürich
Switzerland

Brief description

The Zeiss 780 upright laser scanning confocal microscope as an upright setup can be used for multicolour fluoerescence imaging as well as for surface analyzis/topography application. The system is equipped with a motorized X-Y stage (135×80) and a stage top incubator (Pecon; temperature, CO₂).

The system is further equipped with a tunable chameleon multi photon laser, 2 conventional PMTs and a high sensitive multi array GaAsP detector which can be used for fast lambda scans. For surface data analyzis the microscope computer is equipped with the Confomap® software.

Technical details

Microscope:

Zeiss AxioImager.Z2

Ilumination:

HXP 120 V (visual observation)
laser lines:
- Diode 405 nm
- Argon: 458, 488, 514 nm
- Solid state: 561 nm
- HeNe: 594 nm
- HeNe: 633 nm
- Chameleon MP laser: 705–980 nm

Objectives:

10x 0.3NA EC Plan-Neofluar Ph1 M27
20x 0.8NA Plan-Apochromat M27
40x 0.75NA EC Plan-Neofluar Ph2 M27
40x/0.75NA W N-Achroplan M27 (water dipping lense)
40x 1.3NA Oil Plan-Neofluar DIC M27
63x 1.4NA Oil Plan-Apochromat DIC M27

Camera / detectors:

2 Quasar PMTs (fluorescence), 1 32PMT GaAsP detector, 1 transmission PMT

Software:

ZEN Black 2012
Confomap

Incubator:

The system is equipped with a stage top incubator including temperature control and CO₂ (Pecon).

Fluorescence settings:

for visual observation DAPI, GFP, mCherry and Cy5 filter sets are available