Zeiss LSM 780

Manufacturer:
Zeiss

Model name:
LSM 780

Location:
ETH centre
Universitätsstr. 2
LFW A32.1

Phone:
2 06 44

external page Direct booking link to PPMS

Instrument contact:

Michael Stebler

ScopeM
Otto-Stern-Weg 3
8093 Zürich
Switzerland

Brief description

With the Zeiss 780 laser scanning confocal microscope you can perform automated longterm multi-position life cell experiments. The system is equipped with a motorized X-Y stage (Märzhäuser 130 x 85 mot P), a piezo stage for fast focussing and with an incubator box (temperature, CO₂).

The system is further equipped with:

2 conventional PMTs and a high sensitive multiarray 32PMT GaAsP detector for fast spectral scanning
a high NA long working distance 40x water immersion objective (LD C-Apochromat 40x/1,1 W Korr M27) NA: 1,1 WD: 620 µm @ coverslip thickness 0.17 mm) which allows confocal imaging of several cell layers
the Zeiss hardware autofocus system (definite focus)
AxioCam MRm Rev.3 FireWire as well as GFP, CFP, YFP, DAPI, RFP and Cy3.5 filter sets for widefield microscopy

Technical details

Microscope:

Zeiss AxioObserver.Z1

Ilumination:

HXP 120 C (visual observation+widefield)
laser lines:
- Diode 405 nm
- Argon: 458, 477, 488, 514 nm
- Solid state: 561 nm
- HeNe: 594 nm
- HeNe: 633 nm

Objectives:

10x 0.3NA EC Plan-Neofluar Ph1 M27
20x 0.5NA EC Plan-Neofluar Ph2 M27
40x 1.1NA Water LD C-Apochromat Korr M27 (DIC)
63x 1.4NA Oil Plan-Apochromat DIC M27

Camera / detectors:

2 PMTs, 1 32PMT GaAsP detector, 1 transmission PMT
AxioCam MRm Rev.3 FireWire (1388 × 1040 pixel; pixel size 6.45 µm × 6.45 µm)

Software:

ZEN Black 2012
You can download the an offline ZEN 2012 light version via www.zeiss.com/zen-lite onto your PC (no Mac version available).

Incubator:

The system is equipped with an full box type incubation including stage incubation and CO₂ gassing (Pecon).

Fluorescence settings:

for visual observation GFP, CFP, YFP, DAPI, RFP and Cy3.5 filter sets are available