Zeiss Spinning Disk

Manufacturer:
Zeiss

Model name:
Spinning Disk

Location:
HPM C14.2

Phone:
3 90 51

external page Direct booking link to PPMS

Instrument contact:

Joachim Hehl

ScopeM
Otto-Stern-Weg 3
8093 Zürich
Switzerland

Brief description

This Nipkow spinning disk setup is meant to be used for fast confocal life cell imaging. It's equipped with an incubator for temperature and CO₂. Its Z-resolution doesn't reach the one of the point scanning confocals but it is capable for much higher aquisition speeds.

It is equipped with an objective coupled piezo stepper, a motorized piezo X-Y stage (ASI MS-2000) and filter combinations for fast multicolor acquisition. It has also a hardware autofocus system (Zeiss Definite Focus). Two EM-CCD cameras allow for high sensitive and simultaneous acquisition of combinations of

blue and green,
green and red
green and far red and
red and far red fluorophores

In addition, it is equipped with a point scanner for FRAP and a ring TIRF device.

Technical details

Microscope:

Zeiss Axio Observer Z1 (inverse)

Contrast Methods:

Brightfield, DIC, Spinning Disk Confocal Fluorescence (ring TIRF, FRAP)

Ilumination (visual):

HXP 120 W mercury short arc bulb

Ilumination (LASER):

DPSS 405 nm 120 mW
DPSS 445 nm 100 mW
DPSS 488 nm 200 mW
DPSS 515 nm 100 mW
Diode 561 nm, 200 mW
640 nm, 150 mW

Objectives:

10x 0.3NA EC "Plan Neofluar" M27
40x 1.2NA Water, "C-Apochromat" M27
63x 1.2NA Water, "C-Apochromat" M27
100x 1.46 Oil, "alpha Plan Apochromat" M27 NA Korr Ph2 LD Plan NeoFluar

Camera / detectors:

2x Photometrics Evolve 512 (Back-illuminated EM-CCD, 512 × 512 pixels, pixel size 16 μm × 16 μm)

Incubation:

The system is equipped with an full box type incubation including stage incubation and CO₂ gassing (Pecon). So far it can handle 35 mm dishes and LabTek chambered coverglasses, other formats are possible.

Software:

VisiVIEW ("Metamorph") including Scan Slide, Multiwell Scanning, FRAP and TIRF

Fluorescence settings reflector turret:
(only for visual inspection)

Fluorescence settings Yokogawa motorized dichroic changer:
(multichannel single camera imaging)

Fluorescence settings Yokogawa motorized emission filter wheel:
( multichannel single camera imaging)

Fluorescence settings Yokogawa manual port switch:
(dual channel dual camera imaging)

In the dual camera mode one can choose manually between two possible beam splitters which has to be exchanged manually (ask ScopeM staff), enables for fast two channel imaging.

FT 510 (emission wavelengths under 510 go to camera1 (Evolve1), wavelengths above 510 go to camera 2 (Evolve2))
FT 560 (emission wavelengths under 560 go to camera1 (Evolve1), wavelengths above 560 go to camera 2 (Evolve2))

At the moment, FT 560 is the active one.

Fluorescence settings Yokogawa manual emission filter changer for camera 2:
(dual channel dual camera imaging)

Dual Camera Example: