Olympus FluoView4000

The whole detector unit (Silicon photomultiplier, GHt A/D sampling and digital signal processing unit) is termed SilVIR detector. Compared to the FLUOVIEW 3000 GaAsP PMT detectors SilVIR detectors show increased sensitivity, increased detector dynamic range and photocounting mode. From the 4 SilVIR detectors used in the FLUOVIEW 4000 two are more sensitive in the wavelength range of blue and green fluorescence dyes (SiPM broad band) and two are more sensitive in the range of red and far red fluorescent dyes (SiPM Red shift type). The detectable fluorescence window ranges from 400-900nm and with the addition of a 730nm laser line multi colour imaging can be further expanded into the near infrared range  (f.i. Cy7, AF750).

Technical details

Microscope:

Olympus IX83 (inverse) fully motorized stage with fluorescence filter sets for DAPI, AF488 and AF568 (for conventional epi-illumination).

Ilumination:

• solid state laser lines: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 640 nm, 730 nm
  
• LED based (CoolLED pE300white) unit for conventional epi-illumination for visual observation

Objectives:

• 10x 0.3NA, UPlanFLN PH1
  
• 20x 0.75NA, UPlanSApo
  
• 30x 1.05NA, Silicon UPlanSApo
  
• 60x 1.3NA, Silicon UPlanSApo
  
• 60x 1.35NA, Oil UPlanSApo
  

Detectors:

• 4 SilVIR Detectors (spectral detection option) and 1 transmission PMT

Stage:

• Olympus IX3-SSU motorized XY stage, travel range 114 x 75 mm

Hardware outofocus:

• Z-drift compensator ZDC-2

Incubator:

• Tokai Hit STXG-IX3WX-SET, Thermobox + stage top incubator, temperature controller, gas mixer for CO₂
  
• sample adaptors for galss slides, chambered coverglass, chamberslide 35 mm dishes, 50/60 mm dishes, well plate
  

Software:

• FV4000 system software (CellSenseFV), CellSens software
  

Contrat methods:

• Brightfield, DIC, Confocal Fluorescence