Zeiss Elyra 7

In SIM mode a lattice pattern is applied for sample illumination. Data processing is required to get a super-resolved image. Depending on the imaging algorithm lateral resolution can be improved down to ~120 nm (classical SIM processing) and further down to ~60 nm (SIM2 processing), axial resolution can be improved down to ~300 nm (classical SIM processing) and ~200 nm (SIM2 processing). Processing algorithms are incorporated in the processing section of the ZEN Blue software.

SMLM can be perfomed in TIRF or HiLo mode and resolution can be improved down to the range of 10–20 nm.

On top the Elyra 7 can be used as a stand alone TIRF setup and can as well be used in SIM Apotome mode with lower objective magnifications for fast enhanced optical sectioning with increased contrast and resolution.

For all mentioned options single or dual camera mode can be used. The system is equipped with two Hamamatsu ORCA-Fusion BT sCMOS cameras connected via the Duolink camera adapter.

The microscope is equipped with full incubation enabling 37 °C and 5 % CO₂ for live cell imaging conditions.

Technical details

• hardware autofocus (definite focus)
• AI sample finder for automatic sample detection
• Leap mode and Burst mode: Leap and Burst modes can be combined both with Lattice SIM and SIM Apotome.
  • Leap mode increases the frame rate by a factor of 3 for 3D image acquisition.
  • After Burst processing max. 255 image frames per second at 512×512 px resolution and 1 ms exposure time are available for 2D data acquisition

Available objectives:

• α Plan-APOCHROMAT 63x/1.46 Oil Corr TIRF
• EC Plan-NEOFLUAR 10x/0.3
• Plan-APOCHROMAT 20x/0.8
• Plan-APOCHROMAT 40x/1.4 Oil DIC (UV) VIS-IR
• Plan-APOCHROMAT 63x/1.4 Oil DIC

Cameras:

• 2x Hamamatsu ORCA-Fusion BT (QE around 95 %) with CoaXPress interface for high acquisition speeds and exposure times down to 1ms

Available laser lines:

• 405 nm / 50 mW
• 488 nm / 500 mW
• 561 nm / 500 mW
• 640 nm / 500 mW

Widefield illumination:

• X-Cite Xylis white light LED

Filter settings: